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Several amplification processes can be used to dramatically enhance the sensitivity of particle-based bioelectronic assays. Treatment of the gold-linked DNA-hybrid assembly with silver ions in the presence of hydroquinone results in catalytic deposition of silver on the gold tracer (acting as a catalyst), leading to a dramatic signal amplification [105]. Wang et al. [106] described a triple amplification bioassay, coupling the carriersphere amplifying units (loaded with numerous gold nanoparticle tags) with the “built-in” pre-concentration of the electrochemical stripping detection and a catalytic enlargement of the multiple gold-particle tags.

Ivanov AN, Evtugyn GA, nyi RE, th K, Budnikov HC (2000) Comparative investigation of electrochemical cholinesterase biosensors for pesticide determination. Anal Chim Acta 404:55–65 30. Perez Pita MT, Reviejo AJ, Manuel De Villena FJ, Pingarron JM (1997) Amperometric selective biosensing of dimethyl- and diethyldithiocarbamates based on inhibition processes in a medium of reversed micelles. Anal Chim Acta 340:89–97 31. Moore E, Pravda M, Guilbault GG (2003) Development of a biosensor for the quantitative detection of 2,4,6-trichloroanisole using screen printed electrodes.

Modified carbon paste electrode. Appl Biochem Biotechnol 136:243–250 28 M. Farré et al. 39. Lucarelli F, Kicela A, Palchetti I, Marrazza G, Mascini M (2002a) Electrochemical DNA biosensor for analysis of wastewater samples. Bioelectrochemistry 58:113–118 40. Mugweru A, Wang B, Rusling J (2004) Voltammetric sensor for oxidized DNA using ultrathin films of osmium and ruthenium metallopolymers. Anal Chem 76:5557–5563 41. Del Carlo M, Di Marcello M, Perugini M, Ponzielli V, Sergi M, Mascini M, Compagnone D (2008) Electrochemical DNA biosensor for polycyclic aromatic hydrocarbon detection.

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