By Jianhua Zhao, Haishan Zeng (auth.), Rongguang Liang (eds.)

This e-book presents an creation to layout of biomedical optical imaging applied sciences and their functions. the most subject matters contain: fluorescence imaging, confocal imaging, micro-endoscope, polarization imaging, hyperspectral imaging, OCT imaging, multimodal imaging and spectroscopic platforms.

Each bankruptcy is written through the realm leaders of the respective fields, and should conceal:

  • principles and barriers of optical imaging technology,
  • system layout and functional implementation for one or particular purposes, together with layout guidance, method configuration, optical layout, part requisites and choice, process optimization and layout examples,
  • recent advances and purposes in biomedical researches and scientific imaging.

This e-book serves as a reference for college students and researchers in optics and biomedical engineering.

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16 Depth-resolved confocal Raman spectra of (a) normal mouse skin and (b) tumor-bearing skin in vivo (average of 24 mice) (Adapted from Wang et al. [41], with permission) 1 Advanced Spectroscopy Technique for Biomedicine 33 the 1;325–1;330 cm 1 range (assigned to nucleic acids) were correlated with the presence of tumors, which can potentially be used as biomarkers for skin cancer detection. 8%). Therefore, the confocal Raman system was able to detect in vivo skin chemical composition variations according to depth and the presence or absence of neoplastic pathology.

However, the improvement of S/N ratio using software binning or combined hardware-software binning is limited because the binning only improves the S/N ratio by as much as the square root of the number of pixels binned together. For weak Raman signals that are readout noise limited, hardware binning is preferred because it improves the S/N ratio linearly with the 1 Advanced Spectroscopy Technique for Biomedicine 25 Fig. 12 Graphic representation of the curve observed in horizontal displacement rounded to pixels (dashed lines).

It consists of the typical five components of a spectroscopy system: the excitation light source, the excitation light delivery unit, the Raman signal collection unit, the spectrograph, and the detection unit, with each component specially designed for easy access to the measurement site and for real-time measurement. 1 Light Source The light sources used in Raman spectroscopy are usually lasers because of their higher power output and narrower bandwidth. The choice of wavelength for Raman measurement depends on the specific application and the spectroscopy properties of the sample.

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