By Harold M. McNair, James M. Miller

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Septa Syringe injection is accomplished through a self-sealing septum, a polymeric silicone with high-temperature stability. Many types of septa are commercially available; some are composed of layers and some have a film of Teflon® on the column side. In selecting one, the properties that should be considered are: high temperature stability, amount of septum “bleed” (decomposition), size, lifetime, and cost. Although lifetimes will vary, most septa will self-seal successfully for 50 injections or more.

Chem. 43, [14], 20A–31A (1971). Ettre, L. , LC-GC 8, 716–724 (1990). Ettre, L. , J. Chromatogr. 112, 1–26 (1975). Ettre, L. , Pure Appl. Chem. 65, 819–872 (1993). See also, Ettre, L. , LC-GC 11, 502 (1993). 10. Dominguez, J. A. , and Diez-Masa, J. , Pure Appl. Chem. 73, 969–981 (2001). 11. , Chrompack International B. , Middleburg, the Netherlands, personal communication, 1996. 12. Berger, T. , Chromatographia 42, 63 (1996). CHAPTER 2 INSTRUMENT OVERVIEW Instrumentation in gas chromatography has continually evolved since the introduction of the first commercial systems in 1954.

0. 5. Strictly speaking, equations (21) and (22) are valid only when the heights of the two peaks are the same, as is shown in Fig. 7. For other ratios of peak heights, the paper by Snyder [2] should be consulted for computer-drawn examples. 2 contains a summary of the most important chromatographic definitions and equations, and a complete list of symbols and acronyms is included in Appendix I. 2 1. ( KC )A = Some Important Chromatographic Equations and Definitions [ A ]S [ A ]M 2. Kc = kβ V 3.

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